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. 2014 Oct 29;9:5025–5040. doi: 10.2147/IJN.S63873

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© 2014 Lindemann et al. This work is published by Dove Medical Press Limited, and licensed under Creative Commons Attribution – Non Commercial (unported, v3.0) License

The full terms of the License are available at http://creativecommons.org/licenses/by-nc/3.0/. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed.

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Cell viability of HNSCC cells UT-SCC-60A (A and C) and UT-SCC-60B (B and D).

Notes: Cell viability was determined using Annexin V-APC/PI staining by flow cytometry after incubation with both types of SPIONs (UL-D and Resovist^®) at different concentrations (0.2 mM, 0.5 mM, 0.9 mM, 1.8 mM Fe) over 120 hours. Data are expressed as means ± standard deviation from three experiments as % of unlabeled control cells. Statistical significance compared to unlabeled cells as controls. *P<0.05; **P<0.01; ***P<0.001. The manufacturer of Resovist^® is Bayer Schering Pharma AG, Leverkusen, Germany.

Abbreviations: APC, allophycocyanin; PI, propidium iodide; HNSCC, head and neck squamous cancer cells; UL-D, University of Luebeck-Dextran coated superparamagnetic nanoparticles; SPION, superparamagnetic iron oxide nanoparticle.