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In vivo NIRF imaging of 4T1 tumor-bearing mice at 4 h postinjection. (a) Targeted group, 64Cu-MSN-800CW-TRC105(Fab); (b) non-targeted group, 64Cu-MSN-800CW; (c) blocking group, 64Cu-MSN-800CW-TRC105(Fab) with a blocking dose of TRC105 (1 mg/mouse). Tumors are indicated by yellow arrowheads. Each mouse was iv injected with MSN nanoconjugates with equal amounts of 800CW dyes (∼400 pmol). All images were acquired by using IVIS spectrum in vivo imaging system (Ex = 745 nm, Em = 800 nm).
