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. 2014 Oct 28;14:286. doi: 10.1186/s12870-014-0286-3

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© Li et al.; licensee BioMed Central Ltd. 2014

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Silencing efficiency and specificity for target genes in silenced plants. Two-week-old tomato seedlings were infiltrated with agrobacteria carrying pTRV2-SlSRs or pTRV2-GUS constructs and leaf samples were collected from pTRV2-SlSRs- and pTRV2-GUS-infitlrated plants at 4 weeks after agroinfiltration. Expression levels of each SlSR genes in targeted and nontargeted SlSR genes-silenced and non-silenced plants were analyzed by qRT-PCR and data obtained were normalized with actin transcript values. Data presented are the means ± SD from three independent experiments.