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. 2014 Oct 28;14:286. doi: 10.1186/s12870-014-0286-3

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© Li et al.; licensee BioMed Central Ltd. 2014

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Silencing of either SlSR1 or SlSR3L activated constitutive defense response. Two-week-old seedlings were infiltrated with agrobacteria carrying pTRV2-SlSR1, pTRV2-SlSR3L or pTRV2-GUS constructs and leaf samples were collected at 2 weeks after VIGS infiltration for detection of H₂O₂ accumulation and analysis of expression of signaling- and defense-related genes. (A) Accumulation of H₂O₂ in leaves of the pTRV2-SlSR1- and pTRV2-SlSR3L-infiltrated plants. (B) Expression of defense-related genes and ROS production-related genes in the pTRV2-SlSR1- and pTRV2-SlSR3L-infiltrated plants. (C) Expression of genes involved in different defense signaling pathways in the pTRV2-SlSR1- and pTRV2-SlSR3L-infiltrated plants. Data presented are the means ± SD from three independent experiments and different letters above the columns indicate significant differences at p < 0.05 level.