Figure 4.

Optimal temperature (A) and thermostability (B) of purified rPoMan5A. The optimal temperature for endo-β-1, 4-mannosidase activity was measured at different temperatures using 0.5% locust bean gum as the substrate. The thermal stability was evaluated as the relative residual activity after incubation without the substrate at different temperatures at pH 4.0. Data are the mean of three replicates, and bars indicate the standard deviation of three replicates.