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Optimal pH and pH stability of purified rPoMan5A. The influence of pH on enzyme activity was determined in different 50 mM buffers using 0.5% locust bean gum as the substrate. The pH stability was shown as the remaining activity after incubation for 30 min at 60°C in buffers at various pH without the substrate. Data are the mean of three replicates, and bars indicate standard deviation of three replicates.
