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TLC analysis of the products after hydrolysis of various mannan polymers (0.5%) by rPoMan5A. The purified enzyme rPoMan5A and 0.5% various substrates (A: locust bean gum, B: konjac glucomannan, C: guar gum) were incubated in 50 mM acetate buffer (pH 4.0) at 60°C. Incubation times (hour or minute) are indicated. S, mannose and manno-oligosaccharides were used as standards.
