Table 3.
Primers used in this study
| Primer name | Primer purpose | Primer sequence | Size (bases) |
|---|---|---|---|
| manA-df | Degenerate primers | CGGGTCTGGGGCTTYAAYGAYGT | 23 |
| manA-dr | GTG CCGTAGTAGATGGTRTTNCCRTCRT | 28 | |
| manA-3-sp3 | Amplify gene of the 3′-end by SEFA-PCR | ACTTTCCATCTGTACNNNNNNNNNTGTGAG | 30 |
| manA-3-sp2 | TGACGACCGAGTCTGACGGAAGCTA | 25 | |
| manA-3-sp1 | CTGGGTCAAGGCGACTAGCCAATAT | 25 | |
| manA-5-sp3 | Amplify gene of the 5′-end by SEFA-PCR | GTGTTGATGGTGGCGNNNNNNNNNGCAAGA | 30 |
| manA-5-sp2 | GGAGATGAGATGTACCGCGAAACCA | 25 | |
| manA-5-sp1 | CCATGTGCTTCGGGTCGAGTGATTT | 25 | |
| manA-f | cDNA cloning primers | ATGACATTGGGATTGACTCAGACGA | 25 |
| manA-r | TCAGATCGCAGCGACATGATT | 21 | |
| manA-ef | Specific expression primers | CGGAATTCCAGGTGGCGGAATATGGCCAGTGT | 32 |
| manA-er | GCTCTAGATAGATCGCAGCGACATGATTCGTCACCA | 36 | |
| 5′AOX | Confirmed primers | GACTGGTTCCAATTGACAAGC | 21 |
| 3′AOX | GCAAATGGCATTCTGACATCC | 21 |
R = A/G, W = A/T, Y = C/T, N = A/T/C/G.