Fig. 3.

ATPase activities of ParA mutant derivatives. The C-terminally His₆-tagged purified ParA derivatives (2 µM) were incubated with ATP and released inorganic phosphate (Pi) was detected spectrophotometrically. The control reactions (no protein added or His₆-ParB alone) were included. Data represent the mean±sd values from at least 10 experiments. Statistical analysis revealed significant differences between WT ParA and two control samples (P<0.01; t-test), but not between different ParA variants (P>0.05; t-test).