Table 1. Plasmids used in this work.
Plasmid | Relevant features | Reference/source |
pAKE600 | oriMB1, oriTRK2, ApR, sacB | El-Sayed et al. (2001) |
pAMB9.37 | pBBRMCS-1 expression vector, lacIq tacp | Ludwiczak et al. (2013) |
pBBR1MCS-1 | IncA/C broad-host-range cloning vector, lacZα–MCS, mob, T7p, T3p, CmR | Kovach et al. (1994) |
pBGS18 | oriMB1, KmR, cloning vector | Spratt et al. (1986) |
pBTM116 | oriMB1, 2μ, ApR, trp1, shuttle vector lexABD | Clontech |
pET28a(+) | oriMB1, KmR, T7p, lacO, His6-tag, T7 tag, expression vector | Novagen |
pET28mod | oriMB1, KmR, T7p, lacO, His6-tag, modified to remove T7 tag | Lukaszewicz et al. (2002) |
pGAD424 | oriMB1, 2μ, ApR, leu2, shuttle vector gal4AD | Clontech |
pKLB1.4 | pGAD424 with gal4AD–parA translational fusion | Bartosik et al. (2004) |
pKLB1.6 | pBTM116 with lexADB–parA translational fusion | Bartosik et al. (2004) |
pKLB2.4 | pGAD424 with gal4AD–parB translational fusion | Bartosik et al. (2004) |
pKLB2.6 | pBTM116 with lexADB–parB translational fusion | Bartosik et al. (2004) |
pKLB28 | pET28mod with T7p–parB transcriptional fusion | Bartosik et al. (2004) |
pKLB60.1 | pAKE600 derivative lacking BamHI site | Lasocki et al. (2007) |
pKLB60.2 | pAKE600 derivative with parA | Lasocki et al. (2007) |
pKLB8.1 | pET28mod with T7p–parA transcriptional fusion | Lasocki et al. (2007) |
pKNT25 | orip15, KmR, lacp–MCS–cyaT25 | Karimova et al. (1998) |
pKT25 | orip15, KmR, lacp–cyaT25–MCS | Karimova et al. (1998) |
pKT25-zip | pKT25 with lacp–cyaT25–GCN4 leucine zipper fragment | Karimova et al. (1998) |
pLKB2 | pKT25 with modified MCS | L. Kusiak* |
pLKB220 | pLKB2 with translationally fused cyaT25–parA | L. Kusiak |
pLKB233 | pLKB2 with translationally fused cyaT25–parB | L. Kusiak |
pLKB4 | pUT18C derivative with modified MCS | L. Kusiak |
pMKB5.1 | pLKB4 with cyaT18–parA translational fusion | M. Kusiak* |
pMKB5.2 | pLKB4 with cyaT18–parB translational fusion | M. Kusiak |
pMKB5.3 | pUT18 with parA–cyaT18 translational fusion | M. Kusiak |
pMKB5.4 | pUT18 with parB–cyaT18 translational fusion | M. Kusiak |
pMKB6.1 | pKNT25 with parA–cyaT25 translational fusion | M. Kusiak |
pMKB6.2 | pKNT25 with parB–cyaT25 translational fusion | M. Kusiak |
pUC18 | oriMB1, ApR, cloning vector | Yanisch-Perron et al. (1985) |
pUT18 | oriColE1, ApR, lacp–MCS–cyaT18 | Karimova et al. (1998) |
pUT18C | oriColE1, ApR, lacp–cyaT18–MCS | Karimova et al. (1998) |
pUT18C-zip | pUT18C with lacp–cyaT18–GCN4 leucine zipper fragment | Karimova et al. (1998) |
MCS, multiple cloning site.
Institute of Biochemistry and Biophysics, Polish Academy of Sciences.