Figure 6.

Superoxide generated by whole electron transport chain and PSI of PsbS-KO and wild-type rice. (a) Thylakoids with whole chain-driven production. (b) Thylakoids with PSI-driven production. NADP⁺ was used as final electron acceptor. Absorbance of NBT (15 μM) at 560 nm was used to detect production. Samples were illuminated at 700 μmol photons m⁻² s⁻¹ for photoinhibition at room temperature. Each sample contained 10 μg chlorophyll per mL. Each point represents mean of at least 4 experiments (SD indicated by bar) and the asterisks denote the results that were significantly different from those in the wild type (*P < 0.05). The statistical significance was evaluated using the Student’s t -test.