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. 2014 Nov 4;4:6899. doi: 10.1038/srep06899

Figure 5. Overexpression of ABCB4 reduces colony formation and proliferation in lung cancer cell lines.

Figure 5

(A). A549, A427 and H322 lung cancer cell lines were transfected with the ABCB4 expression construct or empty vector (pEGFP-C1), selected with G418 for 28 days and Giemsa stained. (B). Experiment was repeated three times and mean colony numbers with according SD are shown for A549, A427 and H322 cells. (C). Cell proliferation of A549 cells was evaluated by bromodeoxyuridine (BrdU) incorporation assay. BrdU incorporation was assessed by an ELISA reader. P values were calculated using two tailed paired t-test.