Table 2.

Most significant miRNAs in Association with Prevalent Atrial Fibrillation in Framingham Offspring Participants

	N		Average Expression			Adjustment
miRN A						Age-, Sex- and miR processing			Multivariable***
	Total	AF Case s	Prevalen t AF (n=153)	No AF (n=2185 )	Fold Change**	OR****	95 % CI	P- value*	OR****	95 % CI	P-value*
miR- 328	2407	150	8.76	7.75	1.13	1.21	1.09 - 1.33	<0.001	1.14	1.02 - 1.28	0.017
miR- 150-5p	2415	151	4.07	3.70	1.10	1.29	1.11 - 1.50	0.001	1.24	1.04 - 1.49	0.018
miR- 331-3p	2396	150	6.54	5.98	1.09	1.18	1.06 - 1.31	0.002	1.14	1.01 - 1.28	0.035
miR- 28-5p	2410	150	13.13	12.10	1.09	1.15	1.05 - 1.26	0.002	1.08	0.98 - 1.19	0.14
miR- 99b-5p	2420	151	13.18	12.32	1.07	1.12	1.04 - 1.20	0.003	1.05	0.96 - 1.16	0.27
miR- 182-5p	1946	126	20.56	21.80	0.94	0.91	0.85 - 0.97	0.003	0.93	0.87 - 1.00	0.04
miR- 31-3p	747	39	24.26	24.85	0.98	0.69	0.54 - 0.89	0.004	0.69	0.54 - 0.90	0.005
miR- 196b- 5p	1829	116	22.92	23.56	0.97	0.84	0.75 - 0.95	0.005	0.83	0.73 - 0.94	0.004
RNU48 -a2	2409	151	3.81	3.46	1.10	1.29	1.08 - 1.53	0.005	1.28	1.03 - 1.59	0.027
miR- 339-5p	2403	151	11.30	10.82	1.04	1.13	1.04 - 1.23	0.006	1.10	1.00 - 1.21	0.05

^*Bonferroni p value cutoff = 0.05/253 miRNAs = 0.0002

^**Fold-change is the change in miRNA expression associated with one cycle-threshold increase. For example, if the Ct increase by 1, the fold-change in expression would be = 0.5.

^***Multivariable adjusted models included age, sex, miR-processing, height, weight, systolic blood pressure, diastolic blood pressure, current smoking, antihypertensive medication use, prevalent heart failure, myocardial infarction, and diabetes mellitus

^****The OR reported represents the ratio of odds resulting from a 1-cycle threshold increase.

AF=atrial fibrillation; OR = odds ratio; miR = miRNA; CI = Confidence Interval