Table 3.
Most significant miRNAs in Association with Incident Atrial Fibrillation in Framingham Offspring Participants
| N | Average expression | Adjustment | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Age-, Sex- and miR processing |
Multivariable*** | ||||||||||
| miRN A |
Total | AF Case s |
Inciden t AF (n=107) |
No AF (n=2185 ) |
Fold Change** |
OR**** | 95 % CI |
P- value* |
OR**** | 95 % CI |
P- value* |
| miR- 152 |
2394 | 106 | 16.37 | 16.88 | 0.97 | 0.87 | 0.79 - 0.95 |
0.001 | 0.89 | 0.81 - 0.98 |
0.015 |
| miR- 375 |
2315 | 102 | 20.74 | 20.97 | 0.99 | 0.83 | 0.72 - 0.96 |
0.001 | 0.82 | 0.71 - 0.95 |
0.007 |
| miR- 221-3p |
2358 | 103 | 14.38 | 14.47 | 0.99 | 0.86 | 0.76 - 0.96 |
0.01 | 0.85 | 0.76 - 0.97 |
0.012 |
| miR- 1274b |
2416 | 107 | 7.14 | 7.60 | 0.94 | 0.81 | 0.69 - 0.96 |
0.013 | 0.83 | 0.71 - 0.99 |
0.033 |
| miR- 134 |
1337 | 61 | 23.01 | 23.63 | 0.97 | 0.86 | 0.76 - 0.97 |
0.013 | 0.85 | 0.75 - 0.96 |
0.009 |
| miR- 200c- 3p |
1831 | 83 | 22.07 | 22.95 | 0.96 | 0.88 | 0.80 - 0.98 |
0.017 | 0.89 | 0.80 - 0.98 |
0.022 |
| miR- 193a- 5p |
2405 | 106 | 16.72 | 17.05 | 0.98 | 0.87 | 0.77 - 0.98 |
0.018 | 0.87 | 0.77 - 0.98 |
0.024 |
| miR- 720 |
2420 | 107 | 5.47 | 5.83 | 0.94 | 0.80 | 0.66 - 0.96 |
0.018 | 0.83 | 0.68 - 1.00 |
0.051 |
| miR- 151a- 3p |
2403 | 105 | 12.44 | 13.10 | 0.95 | 0.87 | 0.78 - 0.98 |
0.019 | 0.88 | 0.78 - 0.99 |
0.031 |
| miR- 29b-2- p |
2330 | 102 | 20.84 | 21.05 | 0.99 | 0.84 | 0.73 - 0.97 |
0.02 | 0.83 | 0.72 - 0.97 |
0.016 |
Bonferroni p value cutoff = 0.05/253 miRNAs = 0.0002
Fold-change is the change in miRNA expression associated with one cycle-threshold increase. For example, if the Ct increase by 1, the fold-change in expression would be = 0.5.
Multivariable adjusted models included age, sex, miR-processing, height, weight, systolic blood pressure, diastolic blood pressure, current smoking, antihypertensive medication use, prevalent heart failure, myocardial infarction, and diabetes mellitus
The OR reported represents the ratio of odds resulting from a 1-cycle threshold increase.
AF=atrial fibrillation; OR = odds ratio; miR = miRNA; CI = Confidence Interval