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. Author manuscript; available in PMC: 2014 Dec 1.
Published in final edited form as: J Mol Cell Cardiol. 2013 Sep 25;65:76–87. doi: 10.1016/j.yjmcc.2013.09.009

Fig. 3.

Fig. 3

Genetic manipulability of aGPVM cultures by Ca2+ channel subunits. A: Control AP averaged across n = 10 syncytial aGPVM monolayers. Steady 2 Hz pacing. D: Corresponding single L-type Ca2+ channel activity from parallel cultures to those in A. Top, exemplar unitary Ba2+ currents evoked by +20-mV voltage step. Bottom, ensemble average current calculated from n = 9 such patches. Decay illustrates VDI. B, E: AP and Ca2+ channel activity following short-term expression of β2a-GFP construct via adenoviral transduction. Format as in A and D. Continued activity throughout voltage step indicates suppression of VDI. C, F: AP and Ca2+ channel activity following long-term expression of β2a-GFP construct via lentiviral transduction. Format as in A and D. Continued activity throughout voltage steps also indicates suppression of VDI.