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. 2013 Dec 25;1(1):39–52.

Figure 3.

Figure 3

PAGE4 is a stress-response protein. (A) CWR22rv1 cells stably expressing PAGE4 (P1) or GFP (G1 and G2) were cultured in medium without glucose (G w/o) for 48 hr, or treated with Adriamycin (ADM) or TNF-α for 24 hr. PAGE4 expression was detected by Western blot. (B) HEK293T cells were transiently transfected with pcDNA3.1-PAGE4-v5 and treated with TNF-α for 0-18 hr. PAGE4 expression was detected by Western blot. (C) Same experiment with (B) but the mRNA levels of PAGE4 was detected by RT-PCR. Data are represented as mean±SD. (D) P1 cells were cultured with proteasome inhibitor MG132, Ubiquitin E1 Inhibitor PYR41, small ubiquitin-related modifi er proteins (SUMOs) inhibitor Ginkgolic Acid (GINK), or DMSO for 24 hr, the expressions of PAGE4 or p53 were detected by Western blot. All experiments were repeated a minimum of three times.