Figure 4.

Overexpression of PAGE4 protects cells from stress-induced apoptosis. A: CWR22rv1 cells were transfected with pCMV6-PAGE4-GFP or pCMV6-GFP and the clones stably expressing PAGE4 were selected. PAGE4 expressing clone (PAGE4-#1), or GFP control clones (GFP-#1 and -#2) were cultured in normal medium with (□) or without (■) glucose for 72 h. Cells were observed under phase-contrast microscope and the representative images are shown. Right panel, cell viability was evaluated by WST-1 assay. B: PAGE4-#1, GFP-#1 and -#2 cells were stained with Propidium Iodide (PI) 48 hr after cultured in medium with or without glucose supplement and subjected to cell cycle analysis. Right panels, cell population in each phase of cell cycle was determined. C: PAGE4-#1, GFP-#1 and -#2 cells were plated in 10-cm dish at 500 cells (medium) or 2000 cells (ADM) per dish, and cultured in normal medium or treated with 1 μg/mL of ADM for 1 hr, respectively. Right panel, colonies were counted after 2-week growing. (□), medium; (■), ADM treatment. Data are represented as mean±SD. All experiments were repeated a minimum of three times. An asterisk indicates P<0.05 compared to control cells.