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TGF-β1 induces phosphorylation of Smad3, p38, and ERK 1/2 in prostate gland fibroblasts. A. Western blots show induction of Smad3, p38 phosphorylation by TGF-β1 treatment in prostate gland fibroblasts; B. TGF-β1 induced ERK 1/2 phosphorylation in prostate gland fibroblasts. Basal phosphorylation was evident and TGF-β1 induced phosphorylation was most apparent at 4 hr, which was inhibited by pretreatment with MEK inhibitor U0126 (4 + U0126) as a control.
