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. 2014 Oct 2;2(3):239–248.

Figure 5.

Figure 5

TGF-β1-induced FGF-2 expression in prostate gland fibroblasts requires ERK1/2 and p38 activity. A. Chemical inhibition of either ERK1/2 phosphorylation (UO126) or p38 activity (SB203580) significantly attenuated TGF-β1-induced FGF-2 mRNA expression in prostate gland fibroblasts relative to control (asterisks). *P < 0.05, N ≥ 3 independent experiments in triplicate. Inhibition of JNK activity (SP600125) resulted in a trend toward elevated mRNA that did not reach statistical significance; B. FGF-2 protein in prostate gland fibroblast cell extracts from cells treated with either TGF-β1 (+) or vehicle controls (-) with or without chemical inhibitors. TGF-β1 induced FGF-2 protein was significantly attenuated (asterisks) in the presence of ERK1/2 and p38 kinase inhibitors, whereas inhibition of JNK resulted in a significant increase in FGF-2 protein (asterisks). *P < 0.05, N=2-4 independent experiments with replicate wells per experiment.