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. 2013 Dec 3;14:349. doi: 10.1186/1471-2105-14-349

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Copyright © 2013 Shivanandan et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License(http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Results of applying the plugin to virus–endosome data from confocal microscopy. (a) Image X of the red channel showing adenovirus serotype 2 (Ad2) tagged with ATTO-647. (b) Image Y of the green channel showing Rab5-EGFP, a marker for endosomes. The results from object detection using MosaicIA are shown as overlaid red circles. Only a single 2D image is used here, and no z-stack. (c,d) Distance distributions obtained after fitting the data with a Plummer and step potential model, respectively. (e,f) The corresponding estimated interaction potentials. The Plummer potential leads to a 4-fold lower fitting error than the step potential.