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. 2013 Nov 27;14:112. doi: 10.1186/1471-2156-14-112

Table 1.

Primer design for PCR analysis of MSTN candidate gene in Atlantic salmon

Gene SNP Primer Sequence (5′-3′) PCR product size (bp) T A (°C)
MSTN-1b
g.1060C > A / g.1086C > T
SaMSTNb23
AAACTGGGCATTCAATGTTCCACCATACCA
567
55
 
 
 
CTGCAGTGGCTATTGGGAAAGCTCGCTAAT
 
 
 
c.3501C > G
SaMSTNb33
ACTTGACGTACGAGCCGAGTTCC
571
50
      TTGCCGCAGCCACACCGACAAC