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. 2014 Nov 4;5:564. doi: 10.3389/fmicb.2014.00564

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Copyright © 2014 Hoyer, Oh, Jones and Cota.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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S. gordonii co-aggregation with C. albicans strains encoding site-directed mutant Als3 alleles. The co-aggregation assay was conducted and analyzed as described above. C. albicans control strains included Als3_LA and null Δals3. Experimental strains produced a mutant Als3 protein on the C. albicans surface. These included mutations in the PBC (Als3-pbc: K59M, A116V, Y301F; and Als3-gk: S170Y; Lin et al., 2014) and in the AFR (Als3-afr: I311S, I313S; Lin et al., 2014). All assays used S. gordonii strain SspB. The full set of comparisons between means is provided as Table 3 in Supplementary Material.