Table 4.

Characterization of endophytes from tall fescue plants in US based on alkaloid profiles and microsatellite variation.

Endophyte genotype	B10 allele sizes (bp)	B11 allele sizes (bp)	PERa	EASa		LOLa		IDTa		No. of plants	States
			perA-A2	dmaW	lpsB	lolC	lolA	idtG	ltmQ
E. coenophiala profile 1-1	161, 170, 184	147, 191	+	+	+	+	+			11	AR, KY, OH, TN, TX, WV
E. coenophiala profile 1-2	139, 170, 184	163, 191	+	+	+	+	+			2	GA
E. coenophiala profile 2-1	152, 161, 178	171, 195	+	+	+	+	+		+	172	AL, AR, GA, KY, MO, MS, NY, OH, OK, PA, SC, TN, TX, WV
E. coenophiala profile 2-2	152, 161, 178	183, 195	+	+	+	+	+		+	3	AL, TX
E. coenophiala profile 2-3	161, 173, 178	171, 195	+	+	+	+	+		+	3	GA (NY)b, PA
E. coenophiala profile 2-4	161, 173, 178	171, 210	+	+	+	+	+		+	4	KY, MS, WV
E. coenophiala profile 3-1	161, 170, 178	155, 163	+	+	+	+	+	+	+	3	PA

^aThe + indicates that a PCR fragment was detected with primers designed to the gene.

^bIdentified out of GA-5 and represents the clone from NY (see Figure 2).