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. Author manuscript; available in PMC: 2015 May 1.
Published in final edited form as: Am J Gastroenterol. 2014 Apr 8;109(5):748–756. doi: 10.1038/ajg.2014.48

Figure 2.

Figure 2

Deamidation of 33-mer in the competitive reactions. 33-mer peptide (25 μM) was incubated with tissue transglutaminase-2 (TG-2) (1 μM) that served as a control reaction. For the competitive reactions, 25 (x1) or, 50 (x2), or 100 μM (x4) elafin was added. Tridegin and insulin were used as positive and negative controls, respectively. The degree of deamidation was analyzed by liquid chromatography-mass spectrometry (LC-MS). Data are represented as mean ± SEM (n=3). *p<0.05 vs control reaction (no elafin), all data points of the enzymatic reaction with 100 μM (x 4) elafin and tridegin are statistically significant (p<0.02) vs. control reaction (no elafin).