Figure 4.
Determination of transduced cell types. The transduced green fluorescent protein (GFP)-positive cells were identified using fluorescent microscopy (original magnification × 63) 30 d after administration of SIV-GFP (4 × 108 TU/mouse) vector to the mouse nose. (A) Ciliated respiratory epithelial cell, (B) neuronal cell in olfactory epithelium, (C) squamous epithelial cell and (D) non-neuronal cell in olfactory epithelium. The central image shows a cross-section through the mouse nose and red boxes indicate regions in mouse nasal epithelium where respective transduced cell types were found. Panels A, B and D were rotated ∼45°, 130° and 180° counter clockwise, respectively, to improve clarity of the figure. Modified from Ref. [42].