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. 2013 Dec 1;13:106. doi: 10.1186/1472-6750-13-106

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Copyright © 2013 Zhang et al.; licensee BioMed Central Ltd.

This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Total protein and purified 7ZFP Active-H in different preparations from Rosetta DE3 bacteria transformed with the pET-28 7ZFP or empty control vectors. (a) Total crude proteins expressing 7ZFP (line-pointed band), Coomassie Blue staining. (b) The expression of 7ZFP Active-D was displayed in Western blot assay using antibody against 6 × Histidine-tag (Lane-1, total protein for pET-28a control vector; Lane-2, total protein for pET-28a-7ZFP vector; Lane-3, the sonicated supernatant for pET-28a-7ZFP vector; Lane-4, the sonicated precipitate for pET-28a-7ZFP vector; Lane-5, 7ZFP purified from the sonicated supernatant using Ni-NTA-Sepharose beads).