Table 1.
Properties of homologous ribonuclease inhibitors
| Species | MW (Da) | No. residues | No. leucine residues (%) | No. cysteine residues (%) | Za | Tmb | GenBank Accession No. |
|---|---|---|---|---|---|---|---|
| Human (H. sapiens) | 49973 | 461 | 92 (20%) | 32 (6.9%) | −22 | 51.7 ± 1.1 | NP_976323 |
| Mouse (M. musculus) | 49816 | 456 | 92 (20%) | 30 (6.6%) | −20 | 48.8 ± 0.5 | NP_001165571 |
| Bovine (B. taurus) | 48850 | 456 | 98 (22%) | 29 (6.4%) | −22 | 52.5 ± 0.9 | NP_001030396 |
| Chicken (G. gallus) | 49846 | 456 | 81 (18%) | 30 (6.6%) | −20 | 52.2 ± 0.4 | NP_001006473 |
| Lizard (A. carolinensis) | 49581 | 456 | 78 (17%) | 29 (6.4%) | −10 | 50.0 ± 0.7 | XP_003214831 |
a
Values of Z refer to the net molecular charge: Arg + Lys − Asp − Glu.
b
Values of Tm are the temperature at the midpoint of thermal denaturation, as determined by incorporation of a hydrophobic dye and quantitation by DSF.