Figure 2. als is required for Wg-dependent target expression.

L3 wing imaginal discs; anterior is to the left, posterior is to the right. als^RNAi expression in the P-compartment of wing imaginal discs (visualized by the co-expression of UAS-GFP, green fluorescence) (A–C′) or in the entire wing pouch (D–G) causes loss of expression of Wg-signaling-dependent target genes—Senseless (A and A′) (line 39000), Distalless (B and B′) (line oligo3), frizzled3 (C and C′) (line oligo3), or wingful (D and E; white arrows indicate the Wg-dependent expression domain of wf at the D/V border which is lost in E.) (line 39000)—and ectopic expression of arrow, which is a negative target (G c.f. F; white arrows indicate the extent of the domain that is free from arrow-lacZ expression) (line oligo3).