Figure 3. als acts in a cell-autonomous manner.

Wg target gene expression (Sens) is affected cell-autonomously upon impaired als function as judged from wing disc analysis at the late L3 stage (A–A″). GFP expression indicates cell clones expressing als^RNAi line oligo3^10UAS (A and A″). Adult wing phenotypes coincide with cell clones that experienced als depletion, as marked by forked wing hairs (B–E). This is reminiscent of adult wing phenotypes obtained when Wg signaling is impaired (Lgs^17E expression) (F–I). Red asterisks (B and F) indicate notches at the wing margin; red arrows (C and G) indicate wing margin notches accompanied by loss of mechano- and sensory bristles; red arrows (D and H) and brackets (E and I) indicate broadened veins.