Figure 5. als functions downstream of the Arm/β-Catenin destruction complex and upstream of activated Armadillo.

Overexpression of the positive components Arrow (A), Dishevelled (L), Arm^S10 (F and Q), or Arm (G and R) and depletion of negative components such as wingful (B), shaggy (C, H, and I), apc (D and J), slimb (E, O, and P), and axin (K) cause phenotypes that reflect ectopic Wg signaling: ectopic sensory bristles (A–G), ectopic veins (A–C), and tissue overgrowth (A, H–K, O, and P), but also reduced organ size upon strong pathway activation (B–F, L, M, and Q) and caused ectopic head cuticle (H–J, L, and M). Co-expression of als^RNAi could suppress these phenotypes in the wing (A′–D′) and the eye (H′–M′). Phenotypes based on slimb^RNAi and Arm^S10 expression could not be suppressed upon als depletion (E′, O′, P′, F′, and Q′). Arm overexpession could not rescue als^RNAi phenotypes (G′ and R′). Heads were photographed from dorsal views (left pictures of H–K′) or lateral views, anterior to the left (right pictures of H–K′, L–R′). als^RNAi lines: oligo3^10UAS (A′–E′) and for the eye analysis; oligo2_5′utr (G′).