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. 2014 Nov 4;12(11):e1001988. doi: 10.1371/journal.pbio.1001988

Figure 6. Als co-localizes and interacts with Ter94.

Figure 6

(A) Western blot analysis after immunoprecipitation (IP) of FLAGAls or Ter94HA. Condition 1: negative control (empty pUAST-attB vector), condition 2: FLAGAls (60 kDa), condition 3: negative control (FLAGGal4, 115 kDa), condition 4: FLAGΔN-Als (40 kDa); Ter94HA (105 kDa) was co-expressed in conditions 2–4. Ter94 binds to full-length Als (condition 2) and ΔN-Als (condition 4), and vice versa. The anti-human-UBXN6 peptide antibody recognizes only the full-length Als, but not ΔN-Als. (B–B″) Transgene expression by nubbin-Gal4 in wing imaginal disc cells shows that FLAGAls co-localizes with Ter94HA. (C and C′) Co-expression of Als-VC and Ter94-VN with GMR-Gal4 in eye imaginal discs results in a Venus YFP fluorescence signal, which demonstrates a physical interaction between Als and Ter94.