Figure 7. Reduction of Armadillo levels upon als depletion.

(A–A″) als depletion (line oligo3^10UAS) in the P-compartment of wing imaginal discs caused reduced Arm protein levels compared to the A-compartment (A′ and A″). CD8-GFP expression marks the cell membranes of the P-compartment, and DAPI staining marks all cellular nuclei. (B and B′) The expression of Arm driven from a transgene by nub-Gal4 is similar to endogenous Arm (cf. Figure S8D′). (C and C′) Upon als depletion (line 39000), Arm levels were strongly reduced. (D–G′) DAPI staining (D′–G′) visualizes the large nuclei of the peripodial membrane (blue, out of the focal plane), which overlies the apical part of wing disc cells including Arm (in the focal plane of D–E′). The expression of Arm^S10-Myc (F–G′) was not altered upon depletion of als (line oligo3^10UAS). (D–E′): apical confocal sections; (F–G′): deeper confocal Z-sections, which show enhanced cytoplasmic Arm^S10 levels.