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. 2014 Nov 4;9(11):e109956. doi: 10.1371/journal.pone.0109956

Figure 1. Inconsistency of ffLuc-eGFP reporter activity in labeled tumors during passages in syngeneic immunocompetent mice.

Figure 1

A, Murine Lewis Lung Carcinoma (LLC) cells were infected with ffLuc-eGFP-expressing lentivirus ex vivo, and subcutaneously transplanted into five syngeneic albino C57BL/6 (c-Brd) mice (#160–164). Reporter activity was monitored by BL imaging of subcutaneous tumor growth (body) and pulmonary metastasis (chest). At day 15 after inoculation, a metastatic BL signal was found in one of the mice (#160 in the lower panel). B, The lung was harvested from #160, and a single glowing metastatic nodule selected using ex vivo imaging (upper panel) was transplanted into five c-Brd mice in the second passage. Imaging results showed that the reporter activity could not be consistently maintained in the resulting palpable tumors (lower panel).