Figure 3. Effect of angiotensin II (ANG II) and des-Arg⁹-bradykinin (DABK) on superoxide anion generation in VSMC.

[A] Bar graph demonstrates the effects of ANG II (0.1 nM), DABK (0.1 nM) and ANG II-DABK co-stimulation for 5 min, in the presence of either vehicle, AT1 antagonist losartan (LOS – 10 µM), B1 receptor antagonist des-Arg⁹-Leu⁸-bradykinin (DAL – 10 µM) or superoxide anion scavenger (TIRON - 1 mM) on superoxide anion generation. Results were expressed as mean±SEM of 5 experiments. *P<0.05 vs vehicle. [B] Bar graph demonstrates the effects of ANG II (100 nM) stimulation for 5 min, in the presence of vehicle, AT1 antagonist, losartan (LOS – 10 µM), or B1 receptor antagonist, des-Arg⁹-Leu⁸-bradykinin (DAL – 10 µM). Results are represented as mean ± SEM of 5 experiments. *P<0.05 vs vehicle.