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. 2014 Nov 4;9(11):e111559. doi: 10.1371/journal.pone.0111559

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© 2014 Young et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Leukocytes were isolated from whole blood collected from BALB/c mice (n = 6) before and 30 min after oral administration of NEC (1 g/kg). A, FACS analyses of leukocytes measuring the expression of cell surface markers: T-cells (CD3, CD4, or CD8), B-cells (B220), or monocytes (F4/80). * p≤0.05 versus time zero. B, Representative FACS analysis of F4/80⁺ expression.