Figure 5. Curcumin inhibits mouse macrophage-migration both in vivo and in vitro.

BALB/c mice (n = 6) were oral gavaged daily with vehicle or NEC (1 g/kg/day) for 5 days before intraperitoneal injection of thioglycollate (3 mL, 3% w/v) to induce inflammation. Following 6 days of continued oral administration of NEC or vehicle daily, peritoneal cells were isolated for flow cytometry. A, Representative images following FACS analysis to measure levels of B-cells (B220⁺), T-cells (CD3⁺) or macrophages (F4/80⁺). B, Cell subtype quantitations detected from total peritoneal cell isolates by FACS analysis. C, RAW 264.7 cells were plated to confluence and streaked. Cell migration was observed after 4 d with and without supplementing curcumin (20 µM) to the medium. Representative images taken from duplicated experiments. Original magnification X 80. *  =  p≤0.05 versus vehicle.