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. 2014 Nov 4;9(11):e111559. doi: 10.1371/journal.pone.0111559

Figure 6. Curcumin labels monocytes and inhibits macrophage migration in human cells.

Figure 6

A, Human cell lines Jurkat, Clone E6-1 and THP-1 were cultured in complete RPMI medium, supplemented with various amounts of curcumin or vehicle, and analyzed by FACS. Representative results are shown. B, THP-1 (top) and primary human monocyte derived macrophages (bottom) were treated with the indicated concentrations of curcumin and cell viability was measured over time by MTT assay. Values are expressed as fold changes relative to initial baseline levels. C, THP-1 cells were differentiated into macrophages and isolated by adherence to culture plates. Scratch assays were performed on adherent cells with or without curcumin to measure cell migration. Representative images (left) and migrated cell counts (right). D, Primary macrophages were isolated from healthy human blood samples (n = 4) and subjected to migration assays with and without curcumin (20 µM) for the indicated time. Representative images (left) and relative fold changes of migrated cells (right) are shown. Original magnification X 40. *  =  p≤0.05 versus vehicle. n.s.  =  not significant versus vehicle.