Figure 2. Antibody-recognition of EcTri a 37, BvTri a 37 and Tri a 37 peptides.

(A) Nitrocellulose-dotted wheat seed extract (WSE), human serum albumin (HSA), EcTri a 37 and BvTri a 37 were tested with sera from ten wheat food allergic patients (1–10), with serum from a non-allergic individual (N), buffer alone (B), with Tri a 37-specific rabbit antibodies (I), the corresponding rabbit pre-immune serum (P) and buffer control (B). (B) Testing of Tri a 37 peptides (P1–P5) as in (A).