Figure 3. Localization of synthetic peptides in the Tri a 37 sequence and surface accessibility prediction.

(A) Amino acid sequence of Tri a 37 showing the N- and C-terminal domain and the originally isolated IgE-reactive fragment (underlined). Cysteins are boxed, basic and acidic amino acids are coloured in red and blue, respectively. Synthetic Tri a 37 peptides P1–P5 are indicated. (B) Surface accessibility plot of the Tri a 37 amino acid sequence. The numbers of the amino acids, the thionin domain (green), the C-terminal acidic extension domain (pink), the synthetic peptides (yellow) are indicated on the x-axis. The calculated accessibility of the residues is indicated on the y-axis. Scores above 5.5 (bold horizontal line) represent regions of high surface accessibility.