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. 2014 Nov 4;9(11):e111515. doi: 10.1371/journal.pone.0111515

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© 2014 Rudra-Ganguly et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(a) BXPC3 cells transduced with either NT shRNA or DDR1 shRNA5 were compared in the clonogenic assay. The cells were seeded in 96 well plates with or without collagen and monitored for colony formation up to 10 days. The clones were visualized by crystal violet staining. (b) and (d) Effect of DDR1 knockdown was evaluated on BXPC3 cell migration with or without collagen using the wound healing assay. The wound closure at different time was photographed. (c) and (e) The rate of wound healing was analyzed by measuring the area with Image J program and plotted. NT shRNA (▽), DDR1 shRNA5 (▾) At study termination, p = 0.0004 (**) for both experiments.