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. 2014 Nov 4;9(11):e111515. doi: 10.1371/journal.pone.0111515

Figure 4. DDR1 knockdown induces upregulation of TGFBI.

Figure 4

(a) Unsupervised hierarchical clustering of BXPC3 cells treated with DDR1 shRNA (D), NT shRNA (N) or untreated parental cells (P). (b) Expression validation of small subset of genes by microarray and qPCR. Mean value of two probes (A_23_P93311 and A_P3264163) was plotted for microarray. Mean value of two sets was plotted for qPCR. DDR1 and TGFBI expression in untreated, NT shRNA or DDR1 shRNA5 treated BXPC3 cells is measured by (c) microarray or (d) qPCR. (e) and (f) Relative DDR1 and TGFBI protein levels in untreated parental, NT shRNA or DDR1 shRNA5 treated BXPC3 cell lysates, supernatants or tumor lysates was measured by ELISA. Statistical significance is reported as *** (p<0.0001), ** (p≤0.005), * (p≤0.01), and NS (not significant).