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. 2014 Nov 4;9(11):e111813. doi: 10.1371/journal.pone.0111813

Figure 2. Tpx2 knockdown significantly affects metastasis but not tumor proliferation.

Figure 2

A) Relative expression levels of Tpx2 in 6DT1 cells as measured by qRT-PCR and western blotting. B) 1×105 6DT1-shTpx2 or -shCtrl cells were orthotopically injected into the mammary fat pad of female FVB mice. Mice were euthanized and lungs dissected and inspected for metastatic nodules on the surface of the lungs 27 days after injection. Asterisks indicate a p-value <0.05. C) Weight of primary tumors dissected from the mammary fat pad of mice described in A. No significant differences (N.S.) were observed. D) The Gene expression-based Outcome for Breast cancer Online (GOBO) database was queried for Tpx2 and distant metastasis-free survival (DMFS) plotted as Kaplan-Meier curves for patients with ER-positive tumors expressing high (blue), intermediate (red), or low (gray) levels of TPX2.