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. 2014 Nov 4;9(11):e111813. doi: 10.1371/journal.pone.0111813

Figure 3. Partial Tpx2 ablation in 6DT1 cells does not change expression levels of Tpx2 network hub genes.

Figure 3

6DT1 mouse mammary carcinoma cells were lentivirally transduced with pLKO-Scrambled (shCtrl), pLKO-Tpx2#1 (shTpx2#1), or pLKO-Tpx2#2 (shTpx2#2) and stable, polyclonal pools generated. qRT PCR was carried out to measure mRNA levels of Tpx2 and eight genes previously identified as ‘hubs’ of the Tpx2 gene expression network [3]. Gene expression levels are displayed relative to levels in shCtrl control cells, error bars represent standard deviations, asterisks indicate p-values <0.05. Only Tpx2 was significantly down regulated in both shTpx2 cell lines (red asterisks).