Figure 2. Opsonophagocytic assay against the homologous strain E. faecium E155.
Opsonophagocytic assay used to test the ability to mediate opsonic killing in the strain E. faecium E155 by antibodies raised against the recombinant proteins at different dilutions. αPpiC (square grid), αPBP5 (horizontal stripes), αLysM (vertical stripes) and αDdcP (rhombic grid), compare with the activity of the preimune rabbit serum (NRS, white bar). Bars represent the mean of data and the error bars represent the standard error of the mean. Statistical significance was determined by ANOVA and Dunnett's Multiple Comparison Test. Comparing killing rates of similar dilutions (i.e. 1∶10) with the NRS, all comparisons were significant at p<0.001 (indicated by asterisk).