Table 1. Bacterial strains and sera used for this study.
Strain or serum | Description* | Reference or source |
Strains | ||
E. faecium E155 | ARE, VRE strain isolated from a patient in the USA (Chicago), CC17 | [35] |
E. faecium E1162 | ARE strain isolated from blood in the Netherlands, CC17 | [72] |
E. faecalis 12030 | isolated from a patient in the USA (Cleveland) | [44] |
E. faecalis type 2 | isolated from a patient in Japan (Sapporo) | [73] |
E. faecalis type 5 | isolated from a patient in Japan (Kobe) | [73] |
E coli M15pRep4 | M15 harboring pREP4 plasmid | (INVITROGEN) |
E. coli M15/pQE30LysM | M15 harboring pREP4 and pQE30LysM plasmids | This study |
E. coli M15/pQE30PpiC | M15 harboring pREP4 and pQE30PpiC plasmids | This study |
E. coli M15/pQE30DdcP | M15 harboring pREP4 and pQE30DdcP plasmids | This study |
E. coli M15/pQE30PBP5 | M15 harboring pREP4 and pQE30PBP5 plasmids | This study |
Sera | ||
NRS | Preimmune sera from rabbit | This study |
αSagA | Rabbit serum raised against the recombinant SagA | [23] |
αLysM | Rabbit serum raised against the recombinant LysM | This study |
αPpiC | Rabbit serum raised against the recombinant PpiC | This study |
αDdcP | Rabbit serum raised against the recombinant DdcP | This study |
αPBP5 | Rabbit serum raised against the recombinant PBP5 | This study |
*ARE, ampicillin resistant enterococci; CC17, clonal linage complex 17; DdcP, D-alanyl-D-alanine carboxypeptidase; PBP5, low affinity penicillin-binding protein 5; PpiC, peptidyl-prolyl cis-trans isomerase; SagA; major secreted antigen; VRE, vancomycin resistant enterococci.