Figure 4. Differential Expression of the MYB Transcription Factor Encoded by the R Locus in Developing Seed Coats of a Stable Black-Seeded Soybean Revertant Line RM30-R* and a Brown-Seeded Line RM38-r.

(A) Soybean seed developmental stages for the RM38-r (brown), RM55-r^m (variegated) and RM30-R* stable black revertant. Encircled in red are the five developmental stages (in mg seed fresh weight) chosen for the RNA-Seq analysis. See Table S1 for the sequence read counts of each sample which ranged from from 30 to 77 million. (B) Expression of Glyma09g36983 in RPKMs plotted against the five stages of seed development in mg seed fresh weight as shown in (A) above. 1: 100–200 mg, 2: 200–300 mg, 3: 300–400 mg, 4: 400–500 mg and 5: 300–400 mg as the seeds enter desiccation. The solid red line represents transcripts derived from seed coats of the RM38-r brown-seeded line without the TgmR* but with a “C”-nt deletion in Exon2. The blue line represent the expression in seed coats of the RM30-R* black revertant line with the TgmR* insertion in Intron2.