Table 3. Differential RNA expression of Glyma09g36983 and related MYB transcription factor genes in seed color isolines.

Cultivar	RM30-R*				RM38-r				T157-iR		UC55-iR
Phenotype	black				brown				imp. black		black
Weight range	3001	3002	4001	4002	3001	3002	4001	4002	300	400	300	400
Glyma09g36983.1	0.32	0.12	1.07	0.38	0.60	1.91	2.19	3.70	3.26	2.55	2.73	5.90
Glyma09g36966.1	0.06	0.04	0.1	0.04	0	0.47	0.4	0.93	0.48	0.17	0.21	0.71
Glyma09g37010.1	0	0	0	0	0	0.02	0	0	0	0	0	0
Glyma18g49670.1	0	0	0	0	0	0	0	0	0	0	0	0

RPKMs are shown for seed coats from seed of two seed fresh weight ranges denoted 300–400 and 400–500 mg as shown in Figure 4A.

Superscripts 1 and 2 for the 400–500 mg seed fresh weight represent two independent RNAseq extractions and sequencing determinations.

Glyma09g36966, Glyma09g37010, and Glyma18g49670 have transcript sequence similarities of 90%, 87%, and 84%, respectively, to Glyma09g36983.

Glyma09g36983 in Phytozome G. max_189 a1_1.1 was previously named Glyma09g36990 in Phytozome G. max_109 a1_1.0.

Glyma09g36966 in Phytozome G. max_189 a1_1.1 was previously named Glyma09g36970 in Phytozome G. max_109 a1_1.0.