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. 2014 Nov 4;9(11):e112010. doi: 10.1371/journal.pone.0112010

Figure 5. Characterization of TcpF wild type, deficient and complemented strains.

Figure 5

(A) E99 was cultured in THB medium overnight and TcpF was detected in cell lysate by Western blot. The mutant and complemented strains were used as control. (B) Phagocytosis of E. faecalis by RAW264.7 cells. E. faecalis E99, SPB03 and SPB04 transformed with plasmid pMV158GFP were incubated with RAW264.7 cells for 45 minutes. Free bacteria were removed by washing and cells were analyzed by flow cytometry (BD Accuri, Ann Arbor, MI). These data are from a representative experiment that was repeated with similar results.