Table 3.

Interleukin-18 (IL-18) knockout and high-fat diet intake, metabolism and motor activity

Measure	IL-18 knockout (n = 7)	Wildtype control (n = 6)
High-fat diet intake, kcal	16.5 ± 1.0 **	11.5 ± 0.6
Cosinor parameters
MESOR, kcal hr−1	0.72 ± 0.04 **	0.50 ± 0.03
Amplitude, kcal hr−1	0.21 ± 0.01	0.21 ± 0.02
Peak, kcal hr−1	0.93 ± 0.05 **	0.72 ± 0.03
Nadir, kcal hr−1	0.51 ± 0.05 **	0.29 ± 0.03
Acrophase, hr	1.2 ± 1.2	2.1 ± 0.5
Goodness-of-fit, r	0.70 ± 0.07	0.76 ± 0.03
Total energy expenditure, kcal/kg0.75	151.5 ± 6.9 *	174.1 ± 5.7
Cosinor parameters
MESOR, kcal (hr*kg0.75)−1	6.59 ± 0.30 *	7.59 ± 0.26
Amplitude, kcal (hr*kg0.75)−1	0.62 ± 0.08 *	0.84 ± 0.05
Peak, kcal (hr*kg0.75)−1	7.22 ± 0.38 *	8.42 ± 0.28
Nadir, kcal (hr*kg0.75)−1	5.97 ± 0.23 *	6.75 ± 0.25
Acrophase, hr	2.3 ± 0.5	3.0 ± 0.5
Goodness-of-fit, r	0.72 ± 0.04	0.78 ± 0.02
Respiratory exchange ratio	0.812 ± 0.005 ***	0.783 ± 0.003
Cosinor parameters
MESOR	0.813 ± 0.005 ***	0.783 ± 0.004
0.783 ± 0.004	0.026 ± 0.003	0.019 ± 0.002
Peak	0.838 ± 0.006 ***	0.803 ± 0.006
Nadir	0.787 ± 0.006 ***	0.764 ± 0.002
Acrophase, hr	−1.8 ± 0.8	−0.1 ± 0.6
Goodness-of-fit, r	0.86 ± 0.04 *	0.73 ± 0.03
Horizontal motor activity, beam breaks	57,879 ± 5189	64,189 ± 4879
Vertical motor activity, beam breaks	15,337 ± 3340	14,215 ± 1566

Daily and cosinor parameters of food intake, energy expenditure, respiratory exchange ratio and motor activity of female Il18^−/− mice and wildtype littermate tested across 23 hr in a CLAMS indirect calorimetry system. Please see Methods for definition of cosinor parameters. Data express M ± SEM. Symbols denote differences from wildtype controls

^*p < 0.05,

^**p < 0.005,

^***p < 0.0005 (Student’s t-test).