Skip to main content
PMC home page
Infection and Immunity logoLink to Infection and Immunity
. 1978 Jul;21(1):286–291. doi: 10.1128/iai.21.1.286-291.1978

Protective ability of Salmonella ribosomal protein and RNA in inbred mice.

M L Misfeldt, W Johnson
PMCID: PMC421988  PMID: 30697

Abstract

Ribosomal vaccines prepared from Salmonella typhimurium were effective immunogens in A/J, C3H/HeDub, and C3H/HeJ mice. Purified ribosomal components were also tested as immunogens in the inbred mice. Protein isolated from a Salmonella ribosomal fraction could protect all three mouse strains. Although purified RNA was shown to be protective for A/J and C3H/HeDub mice, it was not protective for C3H/HeJ mice. Protective immunity could be induced in A/J and C3H/HeDub mice by various immunostimulants. Immunity in C3H/HeJ mice, however, could only be induced by Salmonella ribosomes or protein isolated from the Salmonella ribosomal fraction.

Full text

PDF
290

Images in this article

288Image
on p.288
289Image
on p.289

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Araujo F. G., Remington J. S. Protection against Toxoplasma gondii in mice immunized with Toxoplasma cell fractions, RNA and synthetic polyribonucleotides. Immunology. 1974 Oct;27(4):711–721. [PMC free article] [PubMed] [Google Scholar]
  2. Chedid L., Parant M., Damais C., Parant F., Juy D., Galelli A. Failure of endotoxin to increase nonspecific resistance to infection of lipopolysaccharide low-responder mice. Infect Immun. 1976 Mar;13(3):722–727. doi: 10.1128/iai.13.3.722-727.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Chiller J. M., Skidmore B. J., Morrison D. C., Weigle W. O. Relationship of the structure of bacterial lipopolysaccharides to its function in mitogenesis and adjuvanticity. Proc Natl Acad Sci U S A. 1973 Jul;70(7):2129–2133. doi: 10.1073/pnas.70.7.2129. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Fogel S., Sypherd P. S. Extraction and isolation of individual ribosomal proteins from Escherichia coli. J Bacteriol. 1968 Aug;96(2):358–364. doi: 10.1128/jb.96.2.358-364.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Hardy S. J., Kurland C. G., Voynow P., Mora G. The ribosomal proteins of Escherichia coli. I. Purification of the 30S ribosomal proteins. Biochemistry. 1969 Jul;8(7):2897–2905. doi: 10.1021/bi00835a031. [DOI] [PubMed] [Google Scholar]
  6. Janda J., Work E. A colorimetric estimation of lipopolysaccharides. FEBS Lett. 1971 Sep 1;16(4):343–345. doi: 10.1016/0014-5793(71)80386-1. [DOI] [PubMed] [Google Scholar]
  7. Johnson W. Ribosomal vaccines. I. Immunogenicity of ribosomal fractions isolated from Salmonella typhimurium and Yersinia pestis. Infect Immun. 1972 Jun;5(6):947–952. doi: 10.1128/iai.5.6.947-952.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Johnson W. Ribosomal vaccines. II. Specificity of the immune response to ribosomal ribonucleic acid and protein isolated from Salmonella typhimurium. Infect Immun. 1973 Sep;8(3):395–400. doi: 10.1128/iai.8.3.395-400.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  10. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  11. Levy L., Merigan T. C. Inhibition of multiplication of Mycobacterium leprae by polyinosinic-polycytidylic acid. Antimicrob Agents Chemother. 1977 Jan;11(1):122–125. doi: 10.1128/aac.11.1.122. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Medina S., Vas S. I., Robson H. G. Effect of nonspecific stimulation on the defense mechanisms of inbred mice. J Immunol. 1975 Jun;114(6):1720–1725. [PubMed] [Google Scholar]
  13. Misfeldt M. L., Johnson W. Role of endotoxin contamination in ribiosomal vaccines prepared from Salmonella typhimurium. Infect Immun. 1977 Jul;17(1):98–104. doi: 10.1128/iai.17.1.98-104.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Misfeldt M. L., Johnson W. Variability of protection in inbred mice induced by a ribosomal vaccine prepared from Salmonella typhimurium. Infect Immun. 1976 Sep;14(3):652–659. doi: 10.1128/iai.14.3.652-659.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Remington J. S., Merigan T. C. Synthetic polyanions protect mice against intracellular bacterial infection. Nature. 1970 Apr 25;226(5243):361–363. doi: 10.1038/226361a0. [DOI] [PubMed] [Google Scholar]
  16. Robson H. G., Vas S. I. Resistance of inbred mice to Salmonella typhimurium. J Infect Dis. 1972 Oct;126(4):378–386. doi: 10.1093/infdis/126.4.378. [DOI] [PubMed] [Google Scholar]
  17. Smith R. A., Bigley N. J. Ribonucleic acid-protein fractions of virulent Salmonella typhimurium as protective immunogens. Infect Immun. 1972 Sep;6(3):377–383. doi: 10.1128/iai.6.3.377-383.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Swendsen C. L., Johnson W. Humoral immunity to Streptococcus pneumoniae induced by a pneumococcal ribosomal protein fraction. Infect Immun. 1976 Aug;14(2):345–354. doi: 10.1128/iai.14.2.345-354.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Venneman M. R., Bigley N. J., Berry L. J. Immunogenicity of Ribonucleic Acid Preparations Obtained from Salmonella typhimurium. Infect Immun. 1970 Jun;1(6):574–582. doi: 10.1128/iai.1.6.574-582.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Venneman M. R., Bigley N. J. Isolation and partial characterization of an immunogenic moiety obtained from Salmonella typhimurium. J Bacteriol. 1969 Oct;100(1):140–148. doi: 10.1128/jb.100.1.140-148.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Venneman M. R. Purification of immunogenically active ribonucleic acid preparations of Salmonella typhimurium: molecular-sieve and anion-exchange chromatography. Infect Immun. 1972 Mar;5(3):269–282. doi: 10.1128/iai.5.3.269-282.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. WARAVDEKAR V. S., SASLAW L. D. A sensitive colorimetric method for the estimation of 2-deoxy sugars with the use of the malonaldehyde-thiobarbituric acid reaction. J Biol Chem. 1959 Aug;234(8):1945–1950. [PubMed] [Google Scholar]
  23. Weinstein M. J., Waitz J. A., Came P. E. Induction of resistance to bacterial infections of mice with poly I-poly C. Nature. 1970 Apr 11;226(5241):170–170. doi: 10.1038/226170a0. [DOI] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES